Tips on finding the Queen:
MB will laugh.
first let me say that a lot of experienced beekeepers laugh at me when I tell them this but when I was a kid I use to win the queen finding contests we had at our annual Northwest Beekeepers Association Picnic @ Big Lake in Skagit County. By the time I was 14 I had graduated to the adult competition and still won.
1. The queen has a favorite spot she likes to go to whenever the hive is disturbed (opened). It is usually near the edge of the brood chamber.
2. A queen doesn't like to stay in the middle of things when the hive is disturbed to the center three or four frames has the least likelyhood of containing the queen. To most natural predators the brood is a more desirable feast than the honey so the queen flees the the densest brood areas.
3. Standing at the back of the hive and remove the third frame from the far left and inspect it for the queen. If not there pull the forth frame and inspect and if unsucessfull pull frame 2. If still unsuccessful switch sides of the hive, pulling the third frame from the far right, etc.
4. You will seldom find her on the honey ring, but start your search along the edge of the honey ring and brood cells.
5. When You've located the queen note the frame on which she was located (you might want to write the location on the up side of the inner top) as that will usually be her location every time the hive is examined.
6. Keep in mind that the queen is going to have her attendants trying to hide her so look for a moving clup, first, rather that and individual bee. The queen will often travel through the hive by crawling under workers working the brood. That moving bump or cluster will usually have the queen under it.