As AllenF says; if you have a laying queen she has done all the mating she's ever going to do. She mates with numerous drones a few days after emerging and receives enough sperm to last her lifetime. The drones in that hive, and other hives, will try to mate with other virgin queens in the local area but most of them will die horny and "unfulfilled." Briefly, the drones fly out, usually in the afternoon, to a Drone Congregation Area (DCA) and wait for virgin queens to enter the area. They then pursue the queen in a cloud of drones and the strongest and fastest get to mate, and then the next strongest and so on, until she's mated with 12-25 or so drones. As with other matings in nature it's "selection of the fittest." As winter approaches the bees will force most drones out of the hive so that the drones are not consuming stores that are needed for brood, the queen, and the workers. ;)