Andy, not all bee vacs are created equal. A couple of things worth mentioning.
One is, with any bee vac, you want the vacuum set to where the suction is just enough to pull them off and into the hose, if it is too great, install a regulator to adjust the air flow. I did this with my first bee vac that I purchased from Western bee supply. Its a powerful vac with a large capacity but I found it a little too powerful so I added a regulator that allowed me to adjust the suction strength.
Second, overcrowding the inner boxes of any bee vac will overheat and overstress bees, leading to a high mortality rate. Vacuum to capacity, then remove the inner box to a cool spot. If its very hot out, I vacuum to less than capacity, leaving a little extra room in the inner boxes, which gives them a little more breathing room.
Treat them as a package, particularly when its very warm out. If they will be in the inner box for a longer than anticipated period, spray (mist) them a little with water, a little sugar water misting is OK also. Sometimes when its very hot out, I'll also put a wet rag or towel over the box as well to cool things down a bit.
Thirdly, only vacuum bees, avoid like the plague vacuuming honey, this will cause the hose lining to become sticky, bees to become sticky and stressed and the environment inside the inner box to become messy and sticky thus stressing bees and leading to a high mortality rate.
One hundred bees and a queen could maybe squeak by for a short time with feeding but the odds of them surviving are slim to none with such few numbers. You need to bolster numbers, which is another subject we can cover next if you like.
...JP