another trick I know works well is if you need quick acceptance of a virgin queen put honey on her, not enough to drown her but get a Q-tip and swab her whole back end good and they will should take her in fast, seen it done a few times, they will clean her in no time and except her, Dwight Porter showed me this trick. of course you will have to handle her to do this so if you are know sure you want to do this then introduce in a nuc.